Italian University is offering the construction of stable mammalian cell lines hyper-producing recombinant proteins to pharmaceutical companies, universities and research centres.

Italian university department of biology and biotechnology is offering the construction of cell lines for the production of recombinant proteins in mammalian cells to be used for structural biology studies. Recombinant proteins are particularly relevant in various fields, from drug production and diagnosis to basic research. The expiration of patents for therapeutic protein drugs opens up opportunities for biosimilars to enter the market since pharmaceutical companies are willing to obtain their own expression systems. The production in mammalian cells, although expensive, is necessary for many proteins whose function is dependent on posttranslational modifications. We developed a mammalian vector-host cell system which allows high production efficiency, thereby decreasing production costs. We offer to the market the construction of stable mammalian cell lines (human or hamster) constitutively expressing any protein of interest. The system is already working very well and has been used successfully for the construction of human and Chinese hamster cell lines hyper-expressing different proteins such as hydroxylase 3 (LH3) and human erythropoietin. The service includes protein characterization through mass spectrometry (MALDI - TOF/TOF), western blotting, quantitative analysis of RNA by RT-PCR, cellular characterization by fluorescent in situ hybridization (FISH) and several additional molecular and cellular biology methods. The University is looking for international partners such as companies, active in the pharmaceutical field, universities and research centres. The types of partnership considered are technical and research cooperation agreements, in order to explore the innovative application, research and development solution on recombinant protein.